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anti-rabbit ccsp/scgb1a1  (Millipore)


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    Structured Review

    Millipore anti-rabbit ccsp/scgb1a1
    Anti Rabbit Ccsp/Scgb1a1, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rabbit+anti-ccsp/horseradish+peroxidase/bio_rxiv__2024__11__13__623500-300-31-33
    Average 90 stars, based on 1 article reviews
    anti-rabbit ccsp/scgb1a1 - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    Labeling:

    Article Title: Predictive modeling provides insight into the clinical heterogeneity associated with TARS1 loss-of-function mutations
    Article Snippet: Primary antibodies used were rabbit anti-CCSP (Sigma-Aldrich, 07-623) 1:1000 and rabbit anti-MUC1 (Abcam, ab15481) 1:100.

    Article Title: Modeling diverse genetic subtypes of lung adenocarcinoma with a next-generation alveolar type 2 organoid platform
    Article Snippet: 400 Immunohistochemistry (IHC) 401 Sectioned organoids or tissues were stained with hematoxylin and eosin (H&E) or IHC 402 stained with the following antibodies: organoids: anti-TTF1 (1:500, Abcam, ab76013), 403 rabbit anti-SFTPC (1:5000, Millipore, ABC99), rabbit anti-Caveolin-1 (1:5000, Thermo, 404 C3237), rabbit anti-CCSP (1:5000, Millipore, 07-623), rabbit anti-Keratin 5 (1:5000, 405 Biolegend, 905501), anti-TTF1 (1:500, Abcam, ab76013); tissues: rabbit anti-TTF1 406 (1:500, Abcam, ab76013), rabbit anti-HMGA2 (1:400, Cell Signaling Technology, 407 #8179).

    Article Title: Club cell CREB regulates the goblet cell transcriptional network and pro-mucin effects of IL-1B
    Article Snippet: Dual labeling for CCSP (rabbit anti-CCSP; 07-623, Millipore Sigma; 1:1000 dilution; 3 h), a marker of club cells, and Creb1 (mouse anti-Creb1; MA1-083, Invitrogen; 1:200 dilution; 3 h) was achieved using the ImmPRESS Duet Double Staining Polymer Kit (MP-7724, Vector Laboratories).

    Article Title: Yap/Taz inhibit goblet cell fate to maintain lung epithelial homeostasis
    Article Snippet: Rabbit anti-CCSP , EMD Millipore , Cat# 07–623; RRID:AB_310759.

    Article Title: Modeling diverse genetic subtypes of lung adenocarcinoma with a next-generation alveolar type 2 organoid platform.
    Article Snippet: Sectioned organoids or tissues were stained with hematoxylin and eosin (H&E) or IHC-stained with the following antibodies: GENES & DEVELOPMENT 945 for organoids, anti-TTF1 (1:500; Abcam ab76013), rabbit antiSFTPC (1:5000; Millipore ABC99), rabbit anti-Caveolin-1 (1:5000; Thermo C3237), rabbit anti-CCSP (1:5000; Millipore 07-623), and rabbit anti-Keratin 5 (1:5000; Biolegend 905501); for tissues, rabbit anti-TTF1 (1:500; Abcam ab76013) and rabbit anti-HMGA2 (1:400; Cell Signaling Technology 8179).

    Article Title: Club cell CREB regulates the goblet cell transcriptional network and pro-mucin effects of IL-1B.
    Article Snippet: Dual labeling for CCSP (rabbit anti-CCSP; 07-623, Millipore Sigma; 1:1000 dilution; 3 h), a marker of club cells, and Creb1 (mouse anti-Creb1; MA1-083, Invitrogen; 1:200 dilution; 3 h) was achieved using the ImmPRESS Duet Double Staining Polymer Kit (MP-7724, Vector Laboratories).

    Article Title: Atypical Protein Kinase C? is Required for Bronchioalveolar Stem Cell Expansion and Lung Tumorigenesis
    Article Snippet: Lung tissue sections were stained using goat anti-SPC (Santa Cruz Biotechnology) and rabbit anti-CCSP (Upstate) antibodies diluted in 1% BSA (Sigma-Aldrich) at 4°C overnight.

    Marker:

    Article Title: Predictive modeling provides insight into the clinical heterogeneity associated with TARS1 loss-of-function mutations
    Article Snippet: Primary antibodies used were rabbit anti-CCSP (Sigma-Aldrich, 07-623) 1:1000 and rabbit anti-MUC1 (Abcam, ab15481) 1:100.

    Article Title: Modeling diverse genetic subtypes of lung adenocarcinoma with a next-generation alveolar type 2 organoid platform
    Article Snippet: 400 Immunohistochemistry (IHC) 401 Sectioned organoids or tissues were stained with hematoxylin and eosin (H&E) or IHC 402 stained with the following antibodies: organoids: anti-TTF1 (1:500, Abcam, ab76013), 403 rabbit anti-SFTPC (1:5000, Millipore, ABC99), rabbit anti-Caveolin-1 (1:5000, Thermo, 404 C3237), rabbit anti-CCSP (1:5000, Millipore, 07-623), rabbit anti-Keratin 5 (1:5000, 405 Biolegend, 905501), anti-TTF1 (1:500, Abcam, ab76013); tissues: rabbit anti-TTF1 406 (1:500, Abcam, ab76013), rabbit anti-HMGA2 (1:400, Cell Signaling Technology, 407 #8179).

    Article Title: Club cell CREB regulates the goblet cell transcriptional network and pro-mucin effects of IL-1B
    Article Snippet: Dual labeling for CCSP (rabbit anti-CCSP; 07-623, Millipore Sigma; 1:1000 dilution; 3 h), a marker of club cells, and Creb1 (mouse anti-Creb1; MA1-083, Invitrogen; 1:200 dilution; 3 h) was achieved using the ImmPRESS Duet Double Staining Polymer Kit (MP-7724, Vector Laboratories).

    Article Title: Yap/Taz inhibit goblet cell fate to maintain lung epithelial homeostasis
    Article Snippet: Rabbit anti-CCSP , EMD Millipore , Cat# 07–623; RRID:AB_310759.

    Article Title: Modeling diverse genetic subtypes of lung adenocarcinoma with a next-generation alveolar type 2 organoid platform.
    Article Snippet: Sectioned organoids or tissues were stained with hematoxylin and eosin (H&E) or IHC-stained with the following antibodies: GENES & DEVELOPMENT 945 for organoids, anti-TTF1 (1:500; Abcam ab76013), rabbit antiSFTPC (1:5000; Millipore ABC99), rabbit anti-Caveolin-1 (1:5000; Thermo C3237), rabbit anti-CCSP (1:5000; Millipore 07-623), and rabbit anti-Keratin 5 (1:5000; Biolegend 905501); for tissues, rabbit anti-TTF1 (1:500; Abcam ab76013) and rabbit anti-HMGA2 (1:400; Cell Signaling Technology 8179).

    Article Title: Club cell CREB regulates the goblet cell transcriptional network and pro-mucin effects of IL-1B.
    Article Snippet: Dual labeling for CCSP (rabbit anti-CCSP; 07-623, Millipore Sigma; 1:1000 dilution; 3 h), a marker of club cells, and Creb1 (mouse anti-Creb1; MA1-083, Invitrogen; 1:200 dilution; 3 h) was achieved using the ImmPRESS Duet Double Staining Polymer Kit (MP-7724, Vector Laboratories).

    Article Title: Atypical Protein Kinase C? is Required for Bronchioalveolar Stem Cell Expansion and Lung Tumorigenesis
    Article Snippet: Lung tissue sections were stained using goat anti-SPC (Santa Cruz Biotechnology) and rabbit anti-CCSP (Upstate) antibodies diluted in 1% BSA (Sigma-Aldrich) at 4°C overnight.

    Double Staining:

    Article Title: Predictive modeling provides insight into the clinical heterogeneity associated with TARS1 loss-of-function mutations
    Article Snippet: Primary antibodies used were rabbit anti-CCSP (Sigma-Aldrich, 07-623) 1:1000 and rabbit anti-MUC1 (Abcam, ab15481) 1:100.

    Article Title: Modeling diverse genetic subtypes of lung adenocarcinoma with a next-generation alveolar type 2 organoid platform
    Article Snippet: 400 Immunohistochemistry (IHC) 401 Sectioned organoids or tissues were stained with hematoxylin and eosin (H&E) or IHC 402 stained with the following antibodies: organoids: anti-TTF1 (1:500, Abcam, ab76013), 403 rabbit anti-SFTPC (1:5000, Millipore, ABC99), rabbit anti-Caveolin-1 (1:5000, Thermo, 404 C3237), rabbit anti-CCSP (1:5000, Millipore, 07-623), rabbit anti-Keratin 5 (1:5000, 405 Biolegend, 905501), anti-TTF1 (1:500, Abcam, ab76013); tissues: rabbit anti-TTF1 406 (1:500, Abcam, ab76013), rabbit anti-HMGA2 (1:400, Cell Signaling Technology, 407 #8179).

    Article Title: Club cell CREB regulates the goblet cell transcriptional network and pro-mucin effects of IL-1B
    Article Snippet: Dual labeling for CCSP (rabbit anti-CCSP; 07-623, Millipore Sigma; 1:1000 dilution; 3 h), a marker of club cells, and Creb1 (mouse anti-Creb1; MA1-083, Invitrogen; 1:200 dilution; 3 h) was achieved using the ImmPRESS Duet Double Staining Polymer Kit (MP-7724, Vector Laboratories).

    Article Title: Yap/Taz inhibit goblet cell fate to maintain lung epithelial homeostasis
    Article Snippet: Rabbit anti-CCSP , EMD Millipore , Cat# 07–623; RRID:AB_310759.

    Article Title: Modeling diverse genetic subtypes of lung adenocarcinoma with a next-generation alveolar type 2 organoid platform.
    Article Snippet: Sectioned organoids or tissues were stained with hematoxylin and eosin (H&E) or IHC-stained with the following antibodies: GENES & DEVELOPMENT 945 for organoids, anti-TTF1 (1:500; Abcam ab76013), rabbit antiSFTPC (1:5000; Millipore ABC99), rabbit anti-Caveolin-1 (1:5000; Thermo C3237), rabbit anti-CCSP (1:5000; Millipore 07-623), and rabbit anti-Keratin 5 (1:5000; Biolegend 905501); for tissues, rabbit anti-TTF1 (1:500; Abcam ab76013) and rabbit anti-HMGA2 (1:400; Cell Signaling Technology 8179).

    Article Title: Club cell CREB regulates the goblet cell transcriptional network and pro-mucin effects of IL-1B.
    Article Snippet: Dual labeling for CCSP (rabbit anti-CCSP; 07-623, Millipore Sigma; 1:1000 dilution; 3 h), a marker of club cells, and Creb1 (mouse anti-Creb1; MA1-083, Invitrogen; 1:200 dilution; 3 h) was achieved using the ImmPRESS Duet Double Staining Polymer Kit (MP-7724, Vector Laboratories).

    Article Title: Atypical Protein Kinase C? is Required for Bronchioalveolar Stem Cell Expansion and Lung Tumorigenesis
    Article Snippet: Lung tissue sections were stained using goat anti-SPC (Santa Cruz Biotechnology) and rabbit anti-CCSP (Upstate) antibodies diluted in 1% BSA (Sigma-Aldrich) at 4°C overnight.

    Polymer:

    Article Title: Predictive modeling provides insight into the clinical heterogeneity associated with TARS1 loss-of-function mutations
    Article Snippet: Primary antibodies used were rabbit anti-CCSP (Sigma-Aldrich, 07-623) 1:1000 and rabbit anti-MUC1 (Abcam, ab15481) 1:100.

    Article Title: Modeling diverse genetic subtypes of lung adenocarcinoma with a next-generation alveolar type 2 organoid platform
    Article Snippet: 400 Immunohistochemistry (IHC) 401 Sectioned organoids or tissues were stained with hematoxylin and eosin (H&E) or IHC 402 stained with the following antibodies: organoids: anti-TTF1 (1:500, Abcam, ab76013), 403 rabbit anti-SFTPC (1:5000, Millipore, ABC99), rabbit anti-Caveolin-1 (1:5000, Thermo, 404 C3237), rabbit anti-CCSP (1:5000, Millipore, 07-623), rabbit anti-Keratin 5 (1:5000, 405 Biolegend, 905501), anti-TTF1 (1:500, Abcam, ab76013); tissues: rabbit anti-TTF1 406 (1:500, Abcam, ab76013), rabbit anti-HMGA2 (1:400, Cell Signaling Technology, 407 #8179).

    Article Title: Club cell CREB regulates the goblet cell transcriptional network and pro-mucin effects of IL-1B
    Article Snippet: Dual labeling for CCSP (rabbit anti-CCSP; 07-623, Millipore Sigma; 1:1000 dilution; 3 h), a marker of club cells, and Creb1 (mouse anti-Creb1; MA1-083, Invitrogen; 1:200 dilution; 3 h) was achieved using the ImmPRESS Duet Double Staining Polymer Kit (MP-7724, Vector Laboratories).

    Article Title: Yap/Taz inhibit goblet cell fate to maintain lung epithelial homeostasis
    Article Snippet: Rabbit anti-CCSP , EMD Millipore , Cat# 07–623; RRID:AB_310759.

    Article Title: Modeling diverse genetic subtypes of lung adenocarcinoma with a next-generation alveolar type 2 organoid platform.
    Article Snippet: Sectioned organoids or tissues were stained with hematoxylin and eosin (H&E) or IHC-stained with the following antibodies: GENES & DEVELOPMENT 945 for organoids, anti-TTF1 (1:500; Abcam ab76013), rabbit antiSFTPC (1:5000; Millipore ABC99), rabbit anti-Caveolin-1 (1:5000; Thermo C3237), rabbit anti-CCSP (1:5000; Millipore 07-623), and rabbit anti-Keratin 5 (1:5000; Biolegend 905501); for tissues, rabbit anti-TTF1 (1:500; Abcam ab76013) and rabbit anti-HMGA2 (1:400; Cell Signaling Technology 8179).

    Article Title: Club cell CREB regulates the goblet cell transcriptional network and pro-mucin effects of IL-1B.
    Article Snippet: Dual labeling for CCSP (rabbit anti-CCSP; 07-623, Millipore Sigma; 1:1000 dilution; 3 h), a marker of club cells, and Creb1 (mouse anti-Creb1; MA1-083, Invitrogen; 1:200 dilution; 3 h) was achieved using the ImmPRESS Duet Double Staining Polymer Kit (MP-7724, Vector Laboratories).

    Article Title: Atypical Protein Kinase C? is Required for Bronchioalveolar Stem Cell Expansion and Lung Tumorigenesis
    Article Snippet: Lung tissue sections were stained using goat anti-SPC (Santa Cruz Biotechnology) and rabbit anti-CCSP (Upstate) antibodies diluted in 1% BSA (Sigma-Aldrich) at 4°C overnight.

    Staining:

    Article Title: Predictive modeling provides insight into the clinical heterogeneity associated with TARS1 loss-of-function mutations
    Article Snippet: Primary antibodies used were rabbit anti-CCSP (Sigma-Aldrich, 07-623) 1:1000 and rabbit anti-MUC1 (Abcam, ab15481) 1:100.

    Article Title: Modeling diverse genetic subtypes of lung adenocarcinoma with a next-generation alveolar type 2 organoid platform
    Article Snippet: 400 Immunohistochemistry (IHC) 401 Sectioned organoids or tissues were stained with hematoxylin and eosin (H&E) or IHC 402 stained with the following antibodies: organoids: anti-TTF1 (1:500, Abcam, ab76013), 403 rabbit anti-SFTPC (1:5000, Millipore, ABC99), rabbit anti-Caveolin-1 (1:5000, Thermo, 404 C3237), rabbit anti-CCSP (1:5000, Millipore, 07-623), rabbit anti-Keratin 5 (1:5000, 405 Biolegend, 905501), anti-TTF1 (1:500, Abcam, ab76013); tissues: rabbit anti-TTF1 406 (1:500, Abcam, ab76013), rabbit anti-HMGA2 (1:400, Cell Signaling Technology, 407 #8179).

    Article Title: Club cell CREB regulates the goblet cell transcriptional network and pro-mucin effects of IL-1B
    Article Snippet: Dual labeling for CCSP (rabbit anti-CCSP; 07-623, Millipore Sigma; 1:1000 dilution; 3 h), a marker of club cells, and Creb1 (mouse anti-Creb1; MA1-083, Invitrogen; 1:200 dilution; 3 h) was achieved using the ImmPRESS Duet Double Staining Polymer Kit (MP-7724, Vector Laboratories).

    Article Title: Yap/Taz inhibit goblet cell fate to maintain lung epithelial homeostasis
    Article Snippet: Rabbit anti-CCSP , EMD Millipore , Cat# 07–623; RRID:AB_310759.

    Article Title: Modeling diverse genetic subtypes of lung adenocarcinoma with a next-generation alveolar type 2 organoid platform.
    Article Snippet: Sectioned organoids or tissues were stained with hematoxylin and eosin (H&E) or IHC-stained with the following antibodies: GENES & DEVELOPMENT 945 for organoids, anti-TTF1 (1:500; Abcam ab76013), rabbit antiSFTPC (1:5000; Millipore ABC99), rabbit anti-Caveolin-1 (1:5000; Thermo C3237), rabbit anti-CCSP (1:5000; Millipore 07-623), and rabbit anti-Keratin 5 (1:5000; Biolegend 905501); for tissues, rabbit anti-TTF1 (1:500; Abcam ab76013) and rabbit anti-HMGA2 (1:400; Cell Signaling Technology 8179).

    Article Title: Club cell CREB regulates the goblet cell transcriptional network and pro-mucin effects of IL-1B.
    Article Snippet: Dual labeling for CCSP (rabbit anti-CCSP; 07-623, Millipore Sigma; 1:1000 dilution; 3 h), a marker of club cells, and Creb1 (mouse anti-Creb1; MA1-083, Invitrogen; 1:200 dilution; 3 h) was achieved using the ImmPRESS Duet Double Staining Polymer Kit (MP-7724, Vector Laboratories).

    Article Title: Atypical Protein Kinase C? is Required for Bronchioalveolar Stem Cell Expansion and Lung Tumorigenesis
    Article Snippet: Lung tissue sections were stained using goat anti-SPC (Santa Cruz Biotechnology) and rabbit anti-CCSP (Upstate) antibodies diluted in 1% BSA (Sigma-Aldrich) at 4°C overnight.

    Immunohistochemistry:

    Article Title: Predictive modeling provides insight into the clinical heterogeneity associated with TARS1 loss-of-function mutations
    Article Snippet: Primary antibodies used were rabbit anti-CCSP (Sigma-Aldrich, 07-623) 1:1000 and rabbit anti-MUC1 (Abcam, ab15481) 1:100.

    Article Title: Modeling diverse genetic subtypes of lung adenocarcinoma with a next-generation alveolar type 2 organoid platform
    Article Snippet: 400 Immunohistochemistry (IHC) 401 Sectioned organoids or tissues were stained with hematoxylin and eosin (H&E) or IHC 402 stained with the following antibodies: organoids: anti-TTF1 (1:500, Abcam, ab76013), 403 rabbit anti-SFTPC (1:5000, Millipore, ABC99), rabbit anti-Caveolin-1 (1:5000, Thermo, 404 C3237), rabbit anti-CCSP (1:5000, Millipore, 07-623), rabbit anti-Keratin 5 (1:5000, 405 Biolegend, 905501), anti-TTF1 (1:500, Abcam, ab76013); tissues: rabbit anti-TTF1 406 (1:500, Abcam, ab76013), rabbit anti-HMGA2 (1:400, Cell Signaling Technology, 407 #8179).

    Article Title: Club cell CREB regulates the goblet cell transcriptional network and pro-mucin effects of IL-1B
    Article Snippet: Dual labeling for CCSP (rabbit anti-CCSP; 07-623, Millipore Sigma; 1:1000 dilution; 3 h), a marker of club cells, and Creb1 (mouse anti-Creb1; MA1-083, Invitrogen; 1:200 dilution; 3 h) was achieved using the ImmPRESS Duet Double Staining Polymer Kit (MP-7724, Vector Laboratories).

    Article Title: Yap/Taz inhibit goblet cell fate to maintain lung epithelial homeostasis
    Article Snippet: Rabbit anti-CCSP , EMD Millipore , Cat# 07–623; RRID:AB_310759.

    Article Title: Modeling diverse genetic subtypes of lung adenocarcinoma with a next-generation alveolar type 2 organoid platform.
    Article Snippet: Sectioned organoids or tissues were stained with hematoxylin and eosin (H&E) or IHC-stained with the following antibodies: GENES & DEVELOPMENT 945 for organoids, anti-TTF1 (1:500; Abcam ab76013), rabbit antiSFTPC (1:5000; Millipore ABC99), rabbit anti-Caveolin-1 (1:5000; Thermo C3237), rabbit anti-CCSP (1:5000; Millipore 07-623), and rabbit anti-Keratin 5 (1:5000; Biolegend 905501); for tissues, rabbit anti-TTF1 (1:500; Abcam ab76013) and rabbit anti-HMGA2 (1:400; Cell Signaling Technology 8179).

    Article Title: Club cell CREB regulates the goblet cell transcriptional network and pro-mucin effects of IL-1B.
    Article Snippet: Dual labeling for CCSP (rabbit anti-CCSP; 07-623, Millipore Sigma; 1:1000 dilution; 3 h), a marker of club cells, and Creb1 (mouse anti-Creb1; MA1-083, Invitrogen; 1:200 dilution; 3 h) was achieved using the ImmPRESS Duet Double Staining Polymer Kit (MP-7724, Vector Laboratories).

    Article Title: Atypical Protein Kinase C? is Required for Bronchioalveolar Stem Cell Expansion and Lung Tumorigenesis
    Article Snippet: Lung tissue sections were stained using goat anti-SPC (Santa Cruz Biotechnology) and rabbit anti-CCSP (Upstate) antibodies diluted in 1% BSA (Sigma-Aldrich) at 4°C overnight.

    Recombinant:

    Article Title: Predictive modeling provides insight into the clinical heterogeneity associated with TARS1 loss-of-function mutations
    Article Snippet: Primary antibodies used were rabbit anti-CCSP (Sigma-Aldrich, 07-623) 1:1000 and rabbit anti-MUC1 (Abcam, ab15481) 1:100.

    Article Title: Modeling diverse genetic subtypes of lung adenocarcinoma with a next-generation alveolar type 2 organoid platform
    Article Snippet: 400 Immunohistochemistry (IHC) 401 Sectioned organoids or tissues were stained with hematoxylin and eosin (H&E) or IHC 402 stained with the following antibodies: organoids: anti-TTF1 (1:500, Abcam, ab76013), 403 rabbit anti-SFTPC (1:5000, Millipore, ABC99), rabbit anti-Caveolin-1 (1:5000, Thermo, 404 C3237), rabbit anti-CCSP (1:5000, Millipore, 07-623), rabbit anti-Keratin 5 (1:5000, 405 Biolegend, 905501), anti-TTF1 (1:500, Abcam, ab76013); tissues: rabbit anti-TTF1 406 (1:500, Abcam, ab76013), rabbit anti-HMGA2 (1:400, Cell Signaling Technology, 407 #8179).

    Article Title: Club cell CREB regulates the goblet cell transcriptional network and pro-mucin effects of IL-1B
    Article Snippet: Dual labeling for CCSP (rabbit anti-CCSP; 07-623, Millipore Sigma; 1:1000 dilution; 3 h), a marker of club cells, and Creb1 (mouse anti-Creb1; MA1-083, Invitrogen; 1:200 dilution; 3 h) was achieved using the ImmPRESS Duet Double Staining Polymer Kit (MP-7724, Vector Laboratories).

    Article Title: Yap/Taz inhibit goblet cell fate to maintain lung epithelial homeostasis
    Article Snippet: Rabbit anti-CCSP , EMD Millipore , Cat# 07–623; RRID:AB_310759.

    Article Title: Modeling diverse genetic subtypes of lung adenocarcinoma with a next-generation alveolar type 2 organoid platform.
    Article Snippet: Sectioned organoids or tissues were stained with hematoxylin and eosin (H&E) or IHC-stained with the following antibodies: GENES & DEVELOPMENT 945 for organoids, anti-TTF1 (1:500; Abcam ab76013), rabbit antiSFTPC (1:5000; Millipore ABC99), rabbit anti-Caveolin-1 (1:5000; Thermo C3237), rabbit anti-CCSP (1:5000; Millipore 07-623), and rabbit anti-Keratin 5 (1:5000; Biolegend 905501); for tissues, rabbit anti-TTF1 (1:500; Abcam ab76013) and rabbit anti-HMGA2 (1:400; Cell Signaling Technology 8179).

    Article Title: Club cell CREB regulates the goblet cell transcriptional network and pro-mucin effects of IL-1B.
    Article Snippet: Dual labeling for CCSP (rabbit anti-CCSP; 07-623, Millipore Sigma; 1:1000 dilution; 3 h), a marker of club cells, and Creb1 (mouse anti-Creb1; MA1-083, Invitrogen; 1:200 dilution; 3 h) was achieved using the ImmPRESS Duet Double Staining Polymer Kit (MP-7724, Vector Laboratories).

    Article Title: Atypical Protein Kinase C? is Required for Bronchioalveolar Stem Cell Expansion and Lung Tumorigenesis
    Article Snippet: Lung tissue sections were stained using goat anti-SPC (Santa Cruz Biotechnology) and rabbit anti-CCSP (Upstate) antibodies diluted in 1% BSA (Sigma-Aldrich) at 4°C overnight.

    Lysis:

    Article Title: Predictive modeling provides insight into the clinical heterogeneity associated with TARS1 loss-of-function mutations
    Article Snippet: Primary antibodies used were rabbit anti-CCSP (Sigma-Aldrich, 07-623) 1:1000 and rabbit anti-MUC1 (Abcam, ab15481) 1:100.

    Article Title: Modeling diverse genetic subtypes of lung adenocarcinoma with a next-generation alveolar type 2 organoid platform
    Article Snippet: 400 Immunohistochemistry (IHC) 401 Sectioned organoids or tissues were stained with hematoxylin and eosin (H&E) or IHC 402 stained with the following antibodies: organoids: anti-TTF1 (1:500, Abcam, ab76013), 403 rabbit anti-SFTPC (1:5000, Millipore, ABC99), rabbit anti-Caveolin-1 (1:5000, Thermo, 404 C3237), rabbit anti-CCSP (1:5000, Millipore, 07-623), rabbit anti-Keratin 5 (1:5000, 405 Biolegend, 905501), anti-TTF1 (1:500, Abcam, ab76013); tissues: rabbit anti-TTF1 406 (1:500, Abcam, ab76013), rabbit anti-HMGA2 (1:400, Cell Signaling Technology, 407 #8179).

    Article Title: Club cell CREB regulates the goblet cell transcriptional network and pro-mucin effects of IL-1B
    Article Snippet: Dual labeling for CCSP (rabbit anti-CCSP; 07-623, Millipore Sigma; 1:1000 dilution; 3 h), a marker of club cells, and Creb1 (mouse anti-Creb1; MA1-083, Invitrogen; 1:200 dilution; 3 h) was achieved using the ImmPRESS Duet Double Staining Polymer Kit (MP-7724, Vector Laboratories).

    Article Title: Yap/Taz inhibit goblet cell fate to maintain lung epithelial homeostasis
    Article Snippet: Rabbit anti-CCSP , EMD Millipore , Cat# 07–623; RRID:AB_310759.

    Article Title: Modeling diverse genetic subtypes of lung adenocarcinoma with a next-generation alveolar type 2 organoid platform.
    Article Snippet: Sectioned organoids or tissues were stained with hematoxylin and eosin (H&E) or IHC-stained with the following antibodies: GENES & DEVELOPMENT 945 for organoids, anti-TTF1 (1:500; Abcam ab76013), rabbit antiSFTPC (1:5000; Millipore ABC99), rabbit anti-Caveolin-1 (1:5000; Thermo C3237), rabbit anti-CCSP (1:5000; Millipore 07-623), and rabbit anti-Keratin 5 (1:5000; Biolegend 905501); for tissues, rabbit anti-TTF1 (1:500; Abcam ab76013) and rabbit anti-HMGA2 (1:400; Cell Signaling Technology 8179).

    Article Title: Club cell CREB regulates the goblet cell transcriptional network and pro-mucin effects of IL-1B.
    Article Snippet: Dual labeling for CCSP (rabbit anti-CCSP; 07-623, Millipore Sigma; 1:1000 dilution; 3 h), a marker of club cells, and Creb1 (mouse anti-Creb1; MA1-083, Invitrogen; 1:200 dilution; 3 h) was achieved using the ImmPRESS Duet Double Staining Polymer Kit (MP-7724, Vector Laboratories).

    Article Title: Atypical Protein Kinase C? is Required for Bronchioalveolar Stem Cell Expansion and Lung Tumorigenesis
    Article Snippet: Lung tissue sections were stained using goat anti-SPC (Santa Cruz Biotechnology) and rabbit anti-CCSP (Upstate) antibodies diluted in 1% BSA (Sigma-Aldrich) at 4°C overnight.

    TUNEL Assay:

    Article Title: Predictive modeling provides insight into the clinical heterogeneity associated with TARS1 loss-of-function mutations
    Article Snippet: Primary antibodies used were rabbit anti-CCSP (Sigma-Aldrich, 07-623) 1:1000 and rabbit anti-MUC1 (Abcam, ab15481) 1:100.

    Article Title: Modeling diverse genetic subtypes of lung adenocarcinoma with a next-generation alveolar type 2 organoid platform
    Article Snippet: 400 Immunohistochemistry (IHC) 401 Sectioned organoids or tissues were stained with hematoxylin and eosin (H&E) or IHC 402 stained with the following antibodies: organoids: anti-TTF1 (1:500, Abcam, ab76013), 403 rabbit anti-SFTPC (1:5000, Millipore, ABC99), rabbit anti-Caveolin-1 (1:5000, Thermo, 404 C3237), rabbit anti-CCSP (1:5000, Millipore, 07-623), rabbit anti-Keratin 5 (1:5000, 405 Biolegend, 905501), anti-TTF1 (1:500, Abcam, ab76013); tissues: rabbit anti-TTF1 406 (1:500, Abcam, ab76013), rabbit anti-HMGA2 (1:400, Cell Signaling Technology, 407 #8179).

    Article Title: Club cell CREB regulates the goblet cell transcriptional network and pro-mucin effects of IL-1B
    Article Snippet: Dual labeling for CCSP (rabbit anti-CCSP; 07-623, Millipore Sigma; 1:1000 dilution; 3 h), a marker of club cells, and Creb1 (mouse anti-Creb1; MA1-083, Invitrogen; 1:200 dilution; 3 h) was achieved using the ImmPRESS Duet Double Staining Polymer Kit (MP-7724, Vector Laboratories).

    Article Title: Yap/Taz inhibit goblet cell fate to maintain lung epithelial homeostasis
    Article Snippet: Rabbit anti-CCSP , EMD Millipore , Cat# 07–623; RRID:AB_310759.

    Article Title: Modeling diverse genetic subtypes of lung adenocarcinoma with a next-generation alveolar type 2 organoid platform.
    Article Snippet: Sectioned organoids or tissues were stained with hematoxylin and eosin (H&E) or IHC-stained with the following antibodies: GENES & DEVELOPMENT 945 for organoids, anti-TTF1 (1:500; Abcam ab76013), rabbit antiSFTPC (1:5000; Millipore ABC99), rabbit anti-Caveolin-1 (1:5000; Thermo C3237), rabbit anti-CCSP (1:5000; Millipore 07-623), and rabbit anti-Keratin 5 (1:5000; Biolegend 905501); for tissues, rabbit anti-TTF1 (1:500; Abcam ab76013) and rabbit anti-HMGA2 (1:400; Cell Signaling Technology 8179).

    Article Title: Club cell CREB regulates the goblet cell transcriptional network and pro-mucin effects of IL-1B.
    Article Snippet: Dual labeling for CCSP (rabbit anti-CCSP; 07-623, Millipore Sigma; 1:1000 dilution; 3 h), a marker of club cells, and Creb1 (mouse anti-Creb1; MA1-083, Invitrogen; 1:200 dilution; 3 h) was achieved using the ImmPRESS Duet Double Staining Polymer Kit (MP-7724, Vector Laboratories).

    Article Title: Atypical Protein Kinase C? is Required for Bronchioalveolar Stem Cell Expansion and Lung Tumorigenesis
    Article Snippet: Lung tissue sections were stained using goat anti-SPC (Santa Cruz Biotechnology) and rabbit anti-CCSP (Upstate) antibodies diluted in 1% BSA (Sigma-Aldrich) at 4°C overnight.

    RNAscope:

    Article Title: Predictive modeling provides insight into the clinical heterogeneity associated with TARS1 loss-of-function mutations
    Article Snippet: Primary antibodies used were rabbit anti-CCSP (Sigma-Aldrich, 07-623) 1:1000 and rabbit anti-MUC1 (Abcam, ab15481) 1:100.

    Article Title: Modeling diverse genetic subtypes of lung adenocarcinoma with a next-generation alveolar type 2 organoid platform
    Article Snippet: 400 Immunohistochemistry (IHC) 401 Sectioned organoids or tissues were stained with hematoxylin and eosin (H&E) or IHC 402 stained with the following antibodies: organoids: anti-TTF1 (1:500, Abcam, ab76013), 403 rabbit anti-SFTPC (1:5000, Millipore, ABC99), rabbit anti-Caveolin-1 (1:5000, Thermo, 404 C3237), rabbit anti-CCSP (1:5000, Millipore, 07-623), rabbit anti-Keratin 5 (1:5000, 405 Biolegend, 905501), anti-TTF1 (1:500, Abcam, ab76013); tissues: rabbit anti-TTF1 406 (1:500, Abcam, ab76013), rabbit anti-HMGA2 (1:400, Cell Signaling Technology, 407 #8179).

    Article Title: Club cell CREB regulates the goblet cell transcriptional network and pro-mucin effects of IL-1B
    Article Snippet: Dual labeling for CCSP (rabbit anti-CCSP; 07-623, Millipore Sigma; 1:1000 dilution; 3 h), a marker of club cells, and Creb1 (mouse anti-Creb1; MA1-083, Invitrogen; 1:200 dilution; 3 h) was achieved using the ImmPRESS Duet Double Staining Polymer Kit (MP-7724, Vector Laboratories).

    Article Title: Yap/Taz inhibit goblet cell fate to maintain lung epithelial homeostasis
    Article Snippet: Rabbit anti-CCSP , EMD Millipore , Cat# 07–623; RRID:AB_310759.

    Article Title: Modeling diverse genetic subtypes of lung adenocarcinoma with a next-generation alveolar type 2 organoid platform.
    Article Snippet: Sectioned organoids or tissues were stained with hematoxylin and eosin (H&E) or IHC-stained with the following antibodies: GENES & DEVELOPMENT 945 for organoids, anti-TTF1 (1:500; Abcam ab76013), rabbit antiSFTPC (1:5000; Millipore ABC99), rabbit anti-Caveolin-1 (1:5000; Thermo C3237), rabbit anti-CCSP (1:5000; Millipore 07-623), and rabbit anti-Keratin 5 (1:5000; Biolegend 905501); for tissues, rabbit anti-TTF1 (1:500; Abcam ab76013) and rabbit anti-HMGA2 (1:400; Cell Signaling Technology 8179).

    Article Title: Club cell CREB regulates the goblet cell transcriptional network and pro-mucin effects of IL-1B.
    Article Snippet: Dual labeling for CCSP (rabbit anti-CCSP; 07-623, Millipore Sigma; 1:1000 dilution; 3 h), a marker of club cells, and Creb1 (mouse anti-Creb1; MA1-083, Invitrogen; 1:200 dilution; 3 h) was achieved using the ImmPRESS Duet Double Staining Polymer Kit (MP-7724, Vector Laboratories).

    Article Title: Atypical Protein Kinase C? is Required for Bronchioalveolar Stem Cell Expansion and Lung Tumorigenesis
    Article Snippet: Lung tissue sections were stained using goat anti-SPC (Santa Cruz Biotechnology) and rabbit anti-CCSP (Upstate) antibodies diluted in 1% BSA (Sigma-Aldrich) at 4°C overnight.

    Positive Control:

    Article Title: Predictive modeling provides insight into the clinical heterogeneity associated with TARS1 loss-of-function mutations
    Article Snippet: Primary antibodies used were rabbit anti-CCSP (Sigma-Aldrich, 07-623) 1:1000 and rabbit anti-MUC1 (Abcam, ab15481) 1:100.

    Article Title: Modeling diverse genetic subtypes of lung adenocarcinoma with a next-generation alveolar type 2 organoid platform
    Article Snippet: 400 Immunohistochemistry (IHC) 401 Sectioned organoids or tissues were stained with hematoxylin and eosin (H&E) or IHC 402 stained with the following antibodies: organoids: anti-TTF1 (1:500, Abcam, ab76013), 403 rabbit anti-SFTPC (1:5000, Millipore, ABC99), rabbit anti-Caveolin-1 (1:5000, Thermo, 404 C3237), rabbit anti-CCSP (1:5000, Millipore, 07-623), rabbit anti-Keratin 5 (1:5000, 405 Biolegend, 905501), anti-TTF1 (1:500, Abcam, ab76013); tissues: rabbit anti-TTF1 406 (1:500, Abcam, ab76013), rabbit anti-HMGA2 (1:400, Cell Signaling Technology, 407 #8179).

    Article Title: Club cell CREB regulates the goblet cell transcriptional network and pro-mucin effects of IL-1B
    Article Snippet: Dual labeling for CCSP (rabbit anti-CCSP; 07-623, Millipore Sigma; 1:1000 dilution; 3 h), a marker of club cells, and Creb1 (mouse anti-Creb1; MA1-083, Invitrogen; 1:200 dilution; 3 h) was achieved using the ImmPRESS Duet Double Staining Polymer Kit (MP-7724, Vector Laboratories).

    Article Title: Yap/Taz inhibit goblet cell fate to maintain lung epithelial homeostasis
    Article Snippet: Rabbit anti-CCSP , EMD Millipore , Cat# 07–623; RRID:AB_310759.

    Article Title: Modeling diverse genetic subtypes of lung adenocarcinoma with a next-generation alveolar type 2 organoid platform.
    Article Snippet: Sectioned organoids or tissues were stained with hematoxylin and eosin (H&E) or IHC-stained with the following antibodies: GENES & DEVELOPMENT 945 for organoids, anti-TTF1 (1:500; Abcam ab76013), rabbit antiSFTPC (1:5000; Millipore ABC99), rabbit anti-Caveolin-1 (1:5000; Thermo C3237), rabbit anti-CCSP (1:5000; Millipore 07-623), and rabbit anti-Keratin 5 (1:5000; Biolegend 905501); for tissues, rabbit anti-TTF1 (1:500; Abcam ab76013) and rabbit anti-HMGA2 (1:400; Cell Signaling Technology 8179).

    Article Title: Club cell CREB regulates the goblet cell transcriptional network and pro-mucin effects of IL-1B.
    Article Snippet: Dual labeling for CCSP (rabbit anti-CCSP; 07-623, Millipore Sigma; 1:1000 dilution; 3 h), a marker of club cells, and Creb1 (mouse anti-Creb1; MA1-083, Invitrogen; 1:200 dilution; 3 h) was achieved using the ImmPRESS Duet Double Staining Polymer Kit (MP-7724, Vector Laboratories).

    Article Title: Atypical Protein Kinase C? is Required for Bronchioalveolar Stem Cell Expansion and Lung Tumorigenesis
    Article Snippet: Lung tissue sections were stained using goat anti-SPC (Santa Cruz Biotechnology) and rabbit anti-CCSP (Upstate) antibodies diluted in 1% BSA (Sigma-Aldrich) at 4°C overnight.

    Negative Control:

    Article Title: Predictive modeling provides insight into the clinical heterogeneity associated with TARS1 loss-of-function mutations
    Article Snippet: Primary antibodies used were rabbit anti-CCSP (Sigma-Aldrich, 07-623) 1:1000 and rabbit anti-MUC1 (Abcam, ab15481) 1:100.

    Article Title: Modeling diverse genetic subtypes of lung adenocarcinoma with a next-generation alveolar type 2 organoid platform
    Article Snippet: 400 Immunohistochemistry (IHC) 401 Sectioned organoids or tissues were stained with hematoxylin and eosin (H&E) or IHC 402 stained with the following antibodies: organoids: anti-TTF1 (1:500, Abcam, ab76013), 403 rabbit anti-SFTPC (1:5000, Millipore, ABC99), rabbit anti-Caveolin-1 (1:5000, Thermo, 404 C3237), rabbit anti-CCSP (1:5000, Millipore, 07-623), rabbit anti-Keratin 5 (1:5000, 405 Biolegend, 905501), anti-TTF1 (1:500, Abcam, ab76013); tissues: rabbit anti-TTF1 406 (1:500, Abcam, ab76013), rabbit anti-HMGA2 (1:400, Cell Signaling Technology, 407 #8179).

    Article Title: Club cell CREB regulates the goblet cell transcriptional network and pro-mucin effects of IL-1B
    Article Snippet: Dual labeling for CCSP (rabbit anti-CCSP; 07-623, Millipore Sigma; 1:1000 dilution; 3 h), a marker of club cells, and Creb1 (mouse anti-Creb1; MA1-083, Invitrogen; 1:200 dilution; 3 h) was achieved using the ImmPRESS Duet Double Staining Polymer Kit (MP-7724, Vector Laboratories).

    Article Title: Yap/Taz inhibit goblet cell fate to maintain lung epithelial homeostasis
    Article Snippet: Rabbit anti-CCSP , EMD Millipore , Cat# 07–623; RRID:AB_310759.

    Article Title: Modeling diverse genetic subtypes of lung adenocarcinoma with a next-generation alveolar type 2 organoid platform.
    Article Snippet: Sectioned organoids or tissues were stained with hematoxylin and eosin (H&E) or IHC-stained with the following antibodies: GENES & DEVELOPMENT 945 for organoids, anti-TTF1 (1:500; Abcam ab76013), rabbit antiSFTPC (1:5000; Millipore ABC99), rabbit anti-Caveolin-1 (1:5000; Thermo C3237), rabbit anti-CCSP (1:5000; Millipore 07-623), and rabbit anti-Keratin 5 (1:5000; Biolegend 905501); for tissues, rabbit anti-TTF1 (1:500; Abcam ab76013) and rabbit anti-HMGA2 (1:400; Cell Signaling Technology 8179).

    Article Title: Club cell CREB regulates the goblet cell transcriptional network and pro-mucin effects of IL-1B.
    Article Snippet: Dual labeling for CCSP (rabbit anti-CCSP; 07-623, Millipore Sigma; 1:1000 dilution; 3 h), a marker of club cells, and Creb1 (mouse anti-Creb1; MA1-083, Invitrogen; 1:200 dilution; 3 h) was achieved using the ImmPRESS Duet Double Staining Polymer Kit (MP-7724, Vector Laboratories).

    Article Title: Atypical Protein Kinase C? is Required for Bronchioalveolar Stem Cell Expansion and Lung Tumorigenesis
    Article Snippet: Lung tissue sections were stained using goat anti-SPC (Santa Cruz Biotechnology) and rabbit anti-CCSP (Upstate) antibodies diluted in 1% BSA (Sigma-Aldrich) at 4°C overnight.

    Transfection:

    Article Title: Predictive modeling provides insight into the clinical heterogeneity associated with TARS1 loss-of-function mutations
    Article Snippet: Primary antibodies used were rabbit anti-CCSP (Sigma-Aldrich, 07-623) 1:1000 and rabbit anti-MUC1 (Abcam, ab15481) 1:100.

    Article Title: Modeling diverse genetic subtypes of lung adenocarcinoma with a next-generation alveolar type 2 organoid platform
    Article Snippet: 400 Immunohistochemistry (IHC) 401 Sectioned organoids or tissues were stained with hematoxylin and eosin (H&E) or IHC 402 stained with the following antibodies: organoids: anti-TTF1 (1:500, Abcam, ab76013), 403 rabbit anti-SFTPC (1:5000, Millipore, ABC99), rabbit anti-Caveolin-1 (1:5000, Thermo, 404 C3237), rabbit anti-CCSP (1:5000, Millipore, 07-623), rabbit anti-Keratin 5 (1:5000, 405 Biolegend, 905501), anti-TTF1 (1:500, Abcam, ab76013); tissues: rabbit anti-TTF1 406 (1:500, Abcam, ab76013), rabbit anti-HMGA2 (1:400, Cell Signaling Technology, 407 #8179).

    Article Title: Club cell CREB regulates the goblet cell transcriptional network and pro-mucin effects of IL-1B
    Article Snippet: Dual labeling for CCSP (rabbit anti-CCSP; 07-623, Millipore Sigma; 1:1000 dilution; 3 h), a marker of club cells, and Creb1 (mouse anti-Creb1; MA1-083, Invitrogen; 1:200 dilution; 3 h) was achieved using the ImmPRESS Duet Double Staining Polymer Kit (MP-7724, Vector Laboratories).

    Article Title: Yap/Taz inhibit goblet cell fate to maintain lung epithelial homeostasis
    Article Snippet: Rabbit anti-CCSP , EMD Millipore , Cat# 07–623; RRID:AB_310759.

    Article Title: Modeling diverse genetic subtypes of lung adenocarcinoma with a next-generation alveolar type 2 organoid platform.
    Article Snippet: Sectioned organoids or tissues were stained with hematoxylin and eosin (H&E) or IHC-stained with the following antibodies: GENES & DEVELOPMENT 945 for organoids, anti-TTF1 (1:500; Abcam ab76013), rabbit antiSFTPC (1:5000; Millipore ABC99), rabbit anti-Caveolin-1 (1:5000; Thermo C3237), rabbit anti-CCSP (1:5000; Millipore 07-623), and rabbit anti-Keratin 5 (1:5000; Biolegend 905501); for tissues, rabbit anti-TTF1 (1:500; Abcam ab76013) and rabbit anti-HMGA2 (1:400; Cell Signaling Technology 8179).

    Article Title: Club cell CREB regulates the goblet cell transcriptional network and pro-mucin effects of IL-1B.
    Article Snippet: Dual labeling for CCSP (rabbit anti-CCSP; 07-623, Millipore Sigma; 1:1000 dilution; 3 h), a marker of club cells, and Creb1 (mouse anti-Creb1; MA1-083, Invitrogen; 1:200 dilution; 3 h) was achieved using the ImmPRESS Duet Double Staining Polymer Kit (MP-7724, Vector Laboratories).

    Article Title: Atypical Protein Kinase C? is Required for Bronchioalveolar Stem Cell Expansion and Lung Tumorigenesis
    Article Snippet: Lung tissue sections were stained using goat anti-SPC (Santa Cruz Biotechnology) and rabbit anti-CCSP (Upstate) antibodies diluted in 1% BSA (Sigma-Aldrich) at 4°C overnight.

    Purification:

    Article Title: Predictive modeling provides insight into the clinical heterogeneity associated with TARS1 loss-of-function mutations
    Article Snippet: Primary antibodies used were rabbit anti-CCSP (Sigma-Aldrich, 07-623) 1:1000 and rabbit anti-MUC1 (Abcam, ab15481) 1:100.

    Article Title: Modeling diverse genetic subtypes of lung adenocarcinoma with a next-generation alveolar type 2 organoid platform
    Article Snippet: 400 Immunohistochemistry (IHC) 401 Sectioned organoids or tissues were stained with hematoxylin and eosin (H&E) or IHC 402 stained with the following antibodies: organoids: anti-TTF1 (1:500, Abcam, ab76013), 403 rabbit anti-SFTPC (1:5000, Millipore, ABC99), rabbit anti-Caveolin-1 (1:5000, Thermo, 404 C3237), rabbit anti-CCSP (1:5000, Millipore, 07-623), rabbit anti-Keratin 5 (1:5000, 405 Biolegend, 905501), anti-TTF1 (1:500, Abcam, ab76013); tissues: rabbit anti-TTF1 406 (1:500, Abcam, ab76013), rabbit anti-HMGA2 (1:400, Cell Signaling Technology, 407 #8179).

    Article Title: Club cell CREB regulates the goblet cell transcriptional network and pro-mucin effects of IL-1B
    Article Snippet: Dual labeling for CCSP (rabbit anti-CCSP; 07-623, Millipore Sigma; 1:1000 dilution; 3 h), a marker of club cells, and Creb1 (mouse anti-Creb1; MA1-083, Invitrogen; 1:200 dilution; 3 h) was achieved using the ImmPRESS Duet Double Staining Polymer Kit (MP-7724, Vector Laboratories).

    Article Title: Yap/Taz inhibit goblet cell fate to maintain lung epithelial homeostasis
    Article Snippet: Rabbit anti-CCSP , EMD Millipore , Cat# 07–623; RRID:AB_310759.

    Article Title: Modeling diverse genetic subtypes of lung adenocarcinoma with a next-generation alveolar type 2 organoid platform.
    Article Snippet: Sectioned organoids or tissues were stained with hematoxylin and eosin (H&E) or IHC-stained with the following antibodies: GENES & DEVELOPMENT 945 for organoids, anti-TTF1 (1:500; Abcam ab76013), rabbit antiSFTPC (1:5000; Millipore ABC99), rabbit anti-Caveolin-1 (1:5000; Thermo C3237), rabbit anti-CCSP (1:5000; Millipore 07-623), and rabbit anti-Keratin 5 (1:5000; Biolegend 905501); for tissues, rabbit anti-TTF1 (1:500; Abcam ab76013) and rabbit anti-HMGA2 (1:400; Cell Signaling Technology 8179).

    Article Title: Club cell CREB regulates the goblet cell transcriptional network and pro-mucin effects of IL-1B.
    Article Snippet: Dual labeling for CCSP (rabbit anti-CCSP; 07-623, Millipore Sigma; 1:1000 dilution; 3 h), a marker of club cells, and Creb1 (mouse anti-Creb1; MA1-083, Invitrogen; 1:200 dilution; 3 h) was achieved using the ImmPRESS Duet Double Staining Polymer Kit (MP-7724, Vector Laboratories).

    Article Title: Atypical Protein Kinase C? is Required for Bronchioalveolar Stem Cell Expansion and Lung Tumorigenesis
    Article Snippet: Lung tissue sections were stained using goat anti-SPC (Santa Cruz Biotechnology) and rabbit anti-CCSP (Upstate) antibodies diluted in 1% BSA (Sigma-Aldrich) at 4°C overnight.

    cDNA Synthesis:

    Article Title: Predictive modeling provides insight into the clinical heterogeneity associated with TARS1 loss-of-function mutations
    Article Snippet: Primary antibodies used were rabbit anti-CCSP (Sigma-Aldrich, 07-623) 1:1000 and rabbit anti-MUC1 (Abcam, ab15481) 1:100.

    Article Title: Modeling diverse genetic subtypes of lung adenocarcinoma with a next-generation alveolar type 2 organoid platform
    Article Snippet: 400 Immunohistochemistry (IHC) 401 Sectioned organoids or tissues were stained with hematoxylin and eosin (H&E) or IHC 402 stained with the following antibodies: organoids: anti-TTF1 (1:500, Abcam, ab76013), 403 rabbit anti-SFTPC (1:5000, Millipore, ABC99), rabbit anti-Caveolin-1 (1:5000, Thermo, 404 C3237), rabbit anti-CCSP (1:5000, Millipore, 07-623), rabbit anti-Keratin 5 (1:5000, 405 Biolegend, 905501), anti-TTF1 (1:500, Abcam, ab76013); tissues: rabbit anti-TTF1 406 (1:500, Abcam, ab76013), rabbit anti-HMGA2 (1:400, Cell Signaling Technology, 407 #8179).

    Article Title: Club cell CREB regulates the goblet cell transcriptional network and pro-mucin effects of IL-1B
    Article Snippet: Dual labeling for CCSP (rabbit anti-CCSP; 07-623, Millipore Sigma; 1:1000 dilution; 3 h), a marker of club cells, and Creb1 (mouse anti-Creb1; MA1-083, Invitrogen; 1:200 dilution; 3 h) was achieved using the ImmPRESS Duet Double Staining Polymer Kit (MP-7724, Vector Laboratories).

    Article Title: Yap/Taz inhibit goblet cell fate to maintain lung epithelial homeostasis
    Article Snippet: Rabbit anti-CCSP , EMD Millipore , Cat# 07–623; RRID:AB_310759.

    Article Title: Modeling diverse genetic subtypes of lung adenocarcinoma with a next-generation alveolar type 2 organoid platform.
    Article Snippet: Sectioned organoids or tissues were stained with hematoxylin and eosin (H&E) or IHC-stained with the following antibodies: GENES & DEVELOPMENT 945 for organoids, anti-TTF1 (1:500; Abcam ab76013), rabbit antiSFTPC (1:5000; Millipore ABC99), rabbit anti-Caveolin-1 (1:5000; Thermo C3237), rabbit anti-CCSP (1:5000; Millipore 07-623), and rabbit anti-Keratin 5 (1:5000; Biolegend 905501); for tissues, rabbit anti-TTF1 (1:500; Abcam ab76013) and rabbit anti-HMGA2 (1:400; Cell Signaling Technology 8179).

    Article Title: Club cell CREB regulates the goblet cell transcriptional network and pro-mucin effects of IL-1B.
    Article Snippet: Dual labeling for CCSP (rabbit anti-CCSP; 07-623, Millipore Sigma; 1:1000 dilution; 3 h), a marker of club cells, and Creb1 (mouse anti-Creb1; MA1-083, Invitrogen; 1:200 dilution; 3 h) was achieved using the ImmPRESS Duet Double Staining Polymer Kit (MP-7724, Vector Laboratories).

    Article Title: Atypical Protein Kinase C? is Required for Bronchioalveolar Stem Cell Expansion and Lung Tumorigenesis
    Article Snippet: Lung tissue sections were stained using goat anti-SPC (Santa Cruz Biotechnology) and rabbit anti-CCSP (Upstate) antibodies diluted in 1% BSA (Sigma-Aldrich) at 4°C overnight.

    SYBR Green Assay:

    Article Title: Predictive modeling provides insight into the clinical heterogeneity associated with TARS1 loss-of-function mutations
    Article Snippet: Primary antibodies used were rabbit anti-CCSP (Sigma-Aldrich, 07-623) 1:1000 and rabbit anti-MUC1 (Abcam, ab15481) 1:100.

    Article Title: Modeling diverse genetic subtypes of lung adenocarcinoma with a next-generation alveolar type 2 organoid platform
    Article Snippet: 400 Immunohistochemistry (IHC) 401 Sectioned organoids or tissues were stained with hematoxylin and eosin (H&E) or IHC 402 stained with the following antibodies: organoids: anti-TTF1 (1:500, Abcam, ab76013), 403 rabbit anti-SFTPC (1:5000, Millipore, ABC99), rabbit anti-Caveolin-1 (1:5000, Thermo, 404 C3237), rabbit anti-CCSP (1:5000, Millipore, 07-623), rabbit anti-Keratin 5 (1:5000, 405 Biolegend, 905501), anti-TTF1 (1:500, Abcam, ab76013); tissues: rabbit anti-TTF1 406 (1:500, Abcam, ab76013), rabbit anti-HMGA2 (1:400, Cell Signaling Technology, 407 #8179).

    Article Title: Club cell CREB regulates the goblet cell transcriptional network and pro-mucin effects of IL-1B
    Article Snippet: Dual labeling for CCSP (rabbit anti-CCSP; 07-623, Millipore Sigma; 1:1000 dilution; 3 h), a marker of club cells, and Creb1 (mouse anti-Creb1; MA1-083, Invitrogen; 1:200 dilution; 3 h) was achieved using the ImmPRESS Duet Double Staining Polymer Kit (MP-7724, Vector Laboratories).

    Article Title: Yap/Taz inhibit goblet cell fate to maintain lung epithelial homeostasis
    Article Snippet: Rabbit anti-CCSP , EMD Millipore , Cat# 07–623; RRID:AB_310759.

    Article Title: Modeling diverse genetic subtypes of lung adenocarcinoma with a next-generation alveolar type 2 organoid platform.
    Article Snippet: Sectioned organoids or tissues were stained with hematoxylin and eosin (H&E) or IHC-stained with the following antibodies: GENES & DEVELOPMENT 945 for organoids, anti-TTF1 (1:500; Abcam ab76013), rabbit antiSFTPC (1:5000; Millipore ABC99), rabbit anti-Caveolin-1 (1:5000; Thermo C3237), rabbit anti-CCSP (1:5000; Millipore 07-623), and rabbit anti-Keratin 5 (1:5000; Biolegend 905501); for tissues, rabbit anti-TTF1 (1:500; Abcam ab76013) and rabbit anti-HMGA2 (1:400; Cell Signaling Technology 8179).

    Article Title: Club cell CREB regulates the goblet cell transcriptional network and pro-mucin effects of IL-1B.
    Article Snippet: Dual labeling for CCSP (rabbit anti-CCSP; 07-623, Millipore Sigma; 1:1000 dilution; 3 h), a marker of club cells, and Creb1 (mouse anti-Creb1; MA1-083, Invitrogen; 1:200 dilution; 3 h) was achieved using the ImmPRESS Duet Double Staining Polymer Kit (MP-7724, Vector Laboratories).

    Article Title: Atypical Protein Kinase C? is Required for Bronchioalveolar Stem Cell Expansion and Lung Tumorigenesis
    Article Snippet: Lung tissue sections were stained using goat anti-SPC (Santa Cruz Biotechnology) and rabbit anti-CCSP (Upstate) antibodies diluted in 1% BSA (Sigma-Aldrich) at 4°C overnight.

    Knock-Out:

    Article Title: Predictive modeling provides insight into the clinical heterogeneity associated with TARS1 loss-of-function mutations
    Article Snippet: Primary antibodies used were rabbit anti-CCSP (Sigma-Aldrich, 07-623) 1:1000 and rabbit anti-MUC1 (Abcam, ab15481) 1:100.

    Article Title: Modeling diverse genetic subtypes of lung adenocarcinoma with a next-generation alveolar type 2 organoid platform
    Article Snippet: 400 Immunohistochemistry (IHC) 401 Sectioned organoids or tissues were stained with hematoxylin and eosin (H&E) or IHC 402 stained with the following antibodies: organoids: anti-TTF1 (1:500, Abcam, ab76013), 403 rabbit anti-SFTPC (1:5000, Millipore, ABC99), rabbit anti-Caveolin-1 (1:5000, Thermo, 404 C3237), rabbit anti-CCSP (1:5000, Millipore, 07-623), rabbit anti-Keratin 5 (1:5000, 405 Biolegend, 905501), anti-TTF1 (1:500, Abcam, ab76013); tissues: rabbit anti-TTF1 406 (1:500, Abcam, ab76013), rabbit anti-HMGA2 (1:400, Cell Signaling Technology, 407 #8179).

    Article Title: Club cell CREB regulates the goblet cell transcriptional network and pro-mucin effects of IL-1B
    Article Snippet: Dual labeling for CCSP (rabbit anti-CCSP; 07-623, Millipore Sigma; 1:1000 dilution; 3 h), a marker of club cells, and Creb1 (mouse anti-Creb1; MA1-083, Invitrogen; 1:200 dilution; 3 h) was achieved using the ImmPRESS Duet Double Staining Polymer Kit (MP-7724, Vector Laboratories).

    Article Title: Yap/Taz inhibit goblet cell fate to maintain lung epithelial homeostasis
    Article Snippet: Rabbit anti-CCSP , EMD Millipore , Cat# 07–623; RRID:AB_310759.

    Article Title: Modeling diverse genetic subtypes of lung adenocarcinoma with a next-generation alveolar type 2 organoid platform.
    Article Snippet: Sectioned organoids or tissues were stained with hematoxylin and eosin (H&E) or IHC-stained with the following antibodies: GENES & DEVELOPMENT 945 for organoids, anti-TTF1 (1:500; Abcam ab76013), rabbit antiSFTPC (1:5000; Millipore ABC99), rabbit anti-Caveolin-1 (1:5000; Thermo C3237), rabbit anti-CCSP (1:5000; Millipore 07-623), and rabbit anti-Keratin 5 (1:5000; Biolegend 905501); for tissues, rabbit anti-TTF1 (1:500; Abcam ab76013) and rabbit anti-HMGA2 (1:400; Cell Signaling Technology 8179).

    Article Title: Club cell CREB regulates the goblet cell transcriptional network and pro-mucin effects of IL-1B.
    Article Snippet: Dual labeling for CCSP (rabbit anti-CCSP; 07-623, Millipore Sigma; 1:1000 dilution; 3 h), a marker of club cells, and Creb1 (mouse anti-Creb1; MA1-083, Invitrogen; 1:200 dilution; 3 h) was achieved using the ImmPRESS Duet Double Staining Polymer Kit (MP-7724, Vector Laboratories).

    Article Title: Atypical Protein Kinase C? is Required for Bronchioalveolar Stem Cell Expansion and Lung Tumorigenesis
    Article Snippet: Lung tissue sections were stained using goat anti-SPC (Santa Cruz Biotechnology) and rabbit anti-CCSP (Upstate) antibodies diluted in 1% BSA (Sigma-Aldrich) at 4°C overnight.

    Microarray:

    Article Title: Predictive modeling provides insight into the clinical heterogeneity associated with TARS1 loss-of-function mutations
    Article Snippet: Primary antibodies used were rabbit anti-CCSP (Sigma-Aldrich, 07-623) 1:1000 and rabbit anti-MUC1 (Abcam, ab15481) 1:100.

    Article Title: Modeling diverse genetic subtypes of lung adenocarcinoma with a next-generation alveolar type 2 organoid platform
    Article Snippet: 400 Immunohistochemistry (IHC) 401 Sectioned organoids or tissues were stained with hematoxylin and eosin (H&E) or IHC 402 stained with the following antibodies: organoids: anti-TTF1 (1:500, Abcam, ab76013), 403 rabbit anti-SFTPC (1:5000, Millipore, ABC99), rabbit anti-Caveolin-1 (1:5000, Thermo, 404 C3237), rabbit anti-CCSP (1:5000, Millipore, 07-623), rabbit anti-Keratin 5 (1:5000, 405 Biolegend, 905501), anti-TTF1 (1:500, Abcam, ab76013); tissues: rabbit anti-TTF1 406 (1:500, Abcam, ab76013), rabbit anti-HMGA2 (1:400, Cell Signaling Technology, 407 #8179).

    Article Title: Club cell CREB regulates the goblet cell transcriptional network and pro-mucin effects of IL-1B
    Article Snippet: Dual labeling for CCSP (rabbit anti-CCSP; 07-623, Millipore Sigma; 1:1000 dilution; 3 h), a marker of club cells, and Creb1 (mouse anti-Creb1; MA1-083, Invitrogen; 1:200 dilution; 3 h) was achieved using the ImmPRESS Duet Double Staining Polymer Kit (MP-7724, Vector Laboratories).

    Article Title: Yap/Taz inhibit goblet cell fate to maintain lung epithelial homeostasis
    Article Snippet: Rabbit anti-CCSP , EMD Millipore , Cat# 07–623; RRID:AB_310759.

    Article Title: Modeling diverse genetic subtypes of lung adenocarcinoma with a next-generation alveolar type 2 organoid platform.
    Article Snippet: Sectioned organoids or tissues were stained with hematoxylin and eosin (H&E) or IHC-stained with the following antibodies: GENES & DEVELOPMENT 945 for organoids, anti-TTF1 (1:500; Abcam ab76013), rabbit antiSFTPC (1:5000; Millipore ABC99), rabbit anti-Caveolin-1 (1:5000; Thermo C3237), rabbit anti-CCSP (1:5000; Millipore 07-623), and rabbit anti-Keratin 5 (1:5000; Biolegend 905501); for tissues, rabbit anti-TTF1 (1:500; Abcam ab76013) and rabbit anti-HMGA2 (1:400; Cell Signaling Technology 8179).

    Article Title: Club cell CREB regulates the goblet cell transcriptional network and pro-mucin effects of IL-1B.
    Article Snippet: Dual labeling for CCSP (rabbit anti-CCSP; 07-623, Millipore Sigma; 1:1000 dilution; 3 h), a marker of club cells, and Creb1 (mouse anti-Creb1; MA1-083, Invitrogen; 1:200 dilution; 3 h) was achieved using the ImmPRESS Duet Double Staining Polymer Kit (MP-7724, Vector Laboratories).

    Article Title: Atypical Protein Kinase C? is Required for Bronchioalveolar Stem Cell Expansion and Lung Tumorigenesis
    Article Snippet: Lung tissue sections were stained using goat anti-SPC (Santa Cruz Biotechnology) and rabbit anti-CCSP (Upstate) antibodies diluted in 1% BSA (Sigma-Aldrich) at 4°C overnight.

    Knockdown:

    Article Title: Predictive modeling provides insight into the clinical heterogeneity associated with TARS1 loss-of-function mutations
    Article Snippet: Primary antibodies used were rabbit anti-CCSP (Sigma-Aldrich, 07-623) 1:1000 and rabbit anti-MUC1 (Abcam, ab15481) 1:100.

    Article Title: Modeling diverse genetic subtypes of lung adenocarcinoma with a next-generation alveolar type 2 organoid platform
    Article Snippet: 400 Immunohistochemistry (IHC) 401 Sectioned organoids or tissues were stained with hematoxylin and eosin (H&E) or IHC 402 stained with the following antibodies: organoids: anti-TTF1 (1:500, Abcam, ab76013), 403 rabbit anti-SFTPC (1:5000, Millipore, ABC99), rabbit anti-Caveolin-1 (1:5000, Thermo, 404 C3237), rabbit anti-CCSP (1:5000, Millipore, 07-623), rabbit anti-Keratin 5 (1:5000, 405 Biolegend, 905501), anti-TTF1 (1:500, Abcam, ab76013); tissues: rabbit anti-TTF1 406 (1:500, Abcam, ab76013), rabbit anti-HMGA2 (1:400, Cell Signaling Technology, 407 #8179).

    Article Title: Club cell CREB regulates the goblet cell transcriptional network and pro-mucin effects of IL-1B
    Article Snippet: Dual labeling for CCSP (rabbit anti-CCSP; 07-623, Millipore Sigma; 1:1000 dilution; 3 h), a marker of club cells, and Creb1 (mouse anti-Creb1; MA1-083, Invitrogen; 1:200 dilution; 3 h) was achieved using the ImmPRESS Duet Double Staining Polymer Kit (MP-7724, Vector Laboratories).

    Article Title: Yap/Taz inhibit goblet cell fate to maintain lung epithelial homeostasis
    Article Snippet: Rabbit anti-CCSP , EMD Millipore , Cat# 07–623; RRID:AB_310759.

    Article Title: Modeling diverse genetic subtypes of lung adenocarcinoma with a next-generation alveolar type 2 organoid platform.
    Article Snippet: Sectioned organoids or tissues were stained with hematoxylin and eosin (H&E) or IHC-stained with the following antibodies: GENES & DEVELOPMENT 945 for organoids, anti-TTF1 (1:500; Abcam ab76013), rabbit antiSFTPC (1:5000; Millipore ABC99), rabbit anti-Caveolin-1 (1:5000; Thermo C3237), rabbit anti-CCSP (1:5000; Millipore 07-623), and rabbit anti-Keratin 5 (1:5000; Biolegend 905501); for tissues, rabbit anti-TTF1 (1:500; Abcam ab76013) and rabbit anti-HMGA2 (1:400; Cell Signaling Technology 8179).

    Article Title: Club cell CREB regulates the goblet cell transcriptional network and pro-mucin effects of IL-1B.
    Article Snippet: Dual labeling for CCSP (rabbit anti-CCSP; 07-623, Millipore Sigma; 1:1000 dilution; 3 h), a marker of club cells, and Creb1 (mouse anti-Creb1; MA1-083, Invitrogen; 1:200 dilution; 3 h) was achieved using the ImmPRESS Duet Double Staining Polymer Kit (MP-7724, Vector Laboratories).

    Article Title: Atypical Protein Kinase C? is Required for Bronchioalveolar Stem Cell Expansion and Lung Tumorigenesis
    Article Snippet: Lung tissue sections were stained using goat anti-SPC (Santa Cruz Biotechnology) and rabbit anti-CCSP (Upstate) antibodies diluted in 1% BSA (Sigma-Aldrich) at 4°C overnight.

    Control:

    Article Title: Predictive modeling provides insight into the clinical heterogeneity associated with TARS1 loss-of-function mutations
    Article Snippet: Primary antibodies used were rabbit anti-CCSP (Sigma-Aldrich, 07-623) 1:1000 and rabbit anti-MUC1 (Abcam, ab15481) 1:100.

    Article Title: Modeling diverse genetic subtypes of lung adenocarcinoma with a next-generation alveolar type 2 organoid platform
    Article Snippet: 400 Immunohistochemistry (IHC) 401 Sectioned organoids or tissues were stained with hematoxylin and eosin (H&E) or IHC 402 stained with the following antibodies: organoids: anti-TTF1 (1:500, Abcam, ab76013), 403 rabbit anti-SFTPC (1:5000, Millipore, ABC99), rabbit anti-Caveolin-1 (1:5000, Thermo, 404 C3237), rabbit anti-CCSP (1:5000, Millipore, 07-623), rabbit anti-Keratin 5 (1:5000, 405 Biolegend, 905501), anti-TTF1 (1:500, Abcam, ab76013); tissues: rabbit anti-TTF1 406 (1:500, Abcam, ab76013), rabbit anti-HMGA2 (1:400, Cell Signaling Technology, 407 #8179).

    Article Title: Club cell CREB regulates the goblet cell transcriptional network and pro-mucin effects of IL-1B
    Article Snippet: Dual labeling for CCSP (rabbit anti-CCSP; 07-623, Millipore Sigma; 1:1000 dilution; 3 h), a marker of club cells, and Creb1 (mouse anti-Creb1; MA1-083, Invitrogen; 1:200 dilution; 3 h) was achieved using the ImmPRESS Duet Double Staining Polymer Kit (MP-7724, Vector Laboratories).

    Article Title: Yap/Taz inhibit goblet cell fate to maintain lung epithelial homeostasis
    Article Snippet: Rabbit anti-CCSP , EMD Millipore , Cat# 07–623; RRID:AB_310759.

    Article Title: Modeling diverse genetic subtypes of lung adenocarcinoma with a next-generation alveolar type 2 organoid platform.
    Article Snippet: Sectioned organoids or tissues were stained with hematoxylin and eosin (H&E) or IHC-stained with the following antibodies: GENES & DEVELOPMENT 945 for organoids, anti-TTF1 (1:500; Abcam ab76013), rabbit antiSFTPC (1:5000; Millipore ABC99), rabbit anti-Caveolin-1 (1:5000; Thermo C3237), rabbit anti-CCSP (1:5000; Millipore 07-623), and rabbit anti-Keratin 5 (1:5000; Biolegend 905501); for tissues, rabbit anti-TTF1 (1:500; Abcam ab76013) and rabbit anti-HMGA2 (1:400; Cell Signaling Technology 8179).

    Article Title: Club cell CREB regulates the goblet cell transcriptional network and pro-mucin effects of IL-1B.
    Article Snippet: Dual labeling for CCSP (rabbit anti-CCSP; 07-623, Millipore Sigma; 1:1000 dilution; 3 h), a marker of club cells, and Creb1 (mouse anti-Creb1; MA1-083, Invitrogen; 1:200 dilution; 3 h) was achieved using the ImmPRESS Duet Double Staining Polymer Kit (MP-7724, Vector Laboratories).

    Article Title: Atypical Protein Kinase C? is Required for Bronchioalveolar Stem Cell Expansion and Lung Tumorigenesis
    Article Snippet: Lung tissue sections were stained using goat anti-SPC (Santa Cruz Biotechnology) and rabbit anti-CCSP (Upstate) antibodies diluted in 1% BSA (Sigma-Aldrich) at 4°C overnight.

    Software:

    Article Title: Predictive modeling provides insight into the clinical heterogeneity associated with TARS1 loss-of-function mutations
    Article Snippet: Primary antibodies used were rabbit anti-CCSP (Sigma-Aldrich, 07-623) 1:1000 and rabbit anti-MUC1 (Abcam, ab15481) 1:100.

    Article Title: Modeling diverse genetic subtypes of lung adenocarcinoma with a next-generation alveolar type 2 organoid platform
    Article Snippet: 400 Immunohistochemistry (IHC) 401 Sectioned organoids or tissues were stained with hematoxylin and eosin (H&E) or IHC 402 stained with the following antibodies: organoids: anti-TTF1 (1:500, Abcam, ab76013), 403 rabbit anti-SFTPC (1:5000, Millipore, ABC99), rabbit anti-Caveolin-1 (1:5000, Thermo, 404 C3237), rabbit anti-CCSP (1:5000, Millipore, 07-623), rabbit anti-Keratin 5 (1:5000, 405 Biolegend, 905501), anti-TTF1 (1:500, Abcam, ab76013); tissues: rabbit anti-TTF1 406 (1:500, Abcam, ab76013), rabbit anti-HMGA2 (1:400, Cell Signaling Technology, 407 #8179).

    Article Title: Club cell CREB regulates the goblet cell transcriptional network and pro-mucin effects of IL-1B
    Article Snippet: Dual labeling for CCSP (rabbit anti-CCSP; 07-623, Millipore Sigma; 1:1000 dilution; 3 h), a marker of club cells, and Creb1 (mouse anti-Creb1; MA1-083, Invitrogen; 1:200 dilution; 3 h) was achieved using the ImmPRESS Duet Double Staining Polymer Kit (MP-7724, Vector Laboratories).

    Article Title: Yap/Taz inhibit goblet cell fate to maintain lung epithelial homeostasis
    Article Snippet: Rabbit anti-CCSP , EMD Millipore , Cat# 07–623; RRID:AB_310759.

    Article Title: Modeling diverse genetic subtypes of lung adenocarcinoma with a next-generation alveolar type 2 organoid platform.
    Article Snippet: Sectioned organoids or tissues were stained with hematoxylin and eosin (H&E) or IHC-stained with the following antibodies: GENES & DEVELOPMENT 945 for organoids, anti-TTF1 (1:500; Abcam ab76013), rabbit antiSFTPC (1:5000; Millipore ABC99), rabbit anti-Caveolin-1 (1:5000; Thermo C3237), rabbit anti-CCSP (1:5000; Millipore 07-623), and rabbit anti-Keratin 5 (1:5000; Biolegend 905501); for tissues, rabbit anti-TTF1 (1:500; Abcam ab76013) and rabbit anti-HMGA2 (1:400; Cell Signaling Technology 8179).

    Article Title: Club cell CREB regulates the goblet cell transcriptional network and pro-mucin effects of IL-1B.
    Article Snippet: Dual labeling for CCSP (rabbit anti-CCSP; 07-623, Millipore Sigma; 1:1000 dilution; 3 h), a marker of club cells, and Creb1 (mouse anti-Creb1; MA1-083, Invitrogen; 1:200 dilution; 3 h) was achieved using the ImmPRESS Duet Double Staining Polymer Kit (MP-7724, Vector Laboratories).

    Article Title: Atypical Protein Kinase C? is Required for Bronchioalveolar Stem Cell Expansion and Lung Tumorigenesis
    Article Snippet: Lung tissue sections were stained using goat anti-SPC (Santa Cruz Biotechnology) and rabbit anti-CCSP (Upstate) antibodies diluted in 1% BSA (Sigma-Aldrich) at 4°C overnight.



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